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We found a close correlation between the log-transformed albumin results (x) and the test strip reflectance results (y): y (%) = 68.2 − 9.26[logx (mg/L)]; Spearman r = −0.825; P <0.001; Fig. This instrument offers the possibility to obtain reflectance readings. Nissell H, Kublickas M, Lunnell NO, Petterson E. Renal function in gravidas with chronic hypertension with and without superimposed preeclampsia. Fenili D, Pirovano B. PRELIEVI DOMICILIARI. 3. The presence of haptoglobin in urine enhances the peroxidase activity of hemoglobin (29)(30). Correlation between RBC counts (x; flow cytometry) and test strip hemoglobin concentration (y) for RBC counts >25 × 106 cells/L (n = 96). Recently, the URISYS 2400 automated urine test strip analyzer (Roche Diagnostics) was introduced. Combining diagnostic information provided by urinary flow cytometry and more quantitative test strip analysis therefore offers a theoretical basis for the development of diagnostic expert systems (11)(12). Fax 32-9-240-4985; e-mail, © 2002 The American Association for Clinical Chemistry, This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (, Cardiovascular Disease in Women: Understanding the Journey, Giant Magnetoresistive Nanosensor Analysis of Circulating Tumor DNA Epidermal Growth Factor Receptor Mutations for Diagnosis and Therapy Response Monitoring, Inflammation in Atherosclerosis—No Longer a Theory, Assessment of multiplex digital droplet RT-PCR as a diagnostic tool for SARS-CoV-2 detection in nasopharyngeal swabs and saliva samples, The IFCC Clinical Application of Cardiac Biomarkers Committee’s Appraisal of the 2020 ESC Guidelines for the Management of Acute Coronary Syndromes in Patients Presenting Without Persistent ST-segment Elevation: Getting Cardiac Troponin Right, Clinical Chemistry Guide to Scientific Writing, Clinical Chemistry Guide to Manuscript Review, https://doi.org/10.1093/clinchem/48.12.2236, https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model, Receive exclusive offers and updates from Oxford Academic, Copyright © 2021 American Association of Clinical Chemistry. Test results, therefore, no longer need to be expressed in an ordinal scale. Reproducibility of protein, leukocyte esterase, hemoglobin, and glucose on the URISYS 2400 analyzer. Serum free light chain analysis and urine immunofixation electrophoresis in patients with multiple myeloma Autori: Nowrousian MR, Brandhorst D, Sammet C et al. The results are displayed in scattergrams, histograms, and as counts per microliter as well as counts per high-power field. Appunti per la prova scritta ed orale dell’esame di inglese, modulo 1, 2 e 3 previsto dal nuovo ordinamento per la facoltà di medicina e chirurgia per gli studenti del triennio biologico. The within- and between-run CVs for protein, leukocyte esterase, hemoglobin, and glucose are summarized in Table 1. Multiple regression analysis on the study population showed no effect of conductivity on test strip leukocyte esterase. <> Correlation between glucose measurements (glucose concentration different from 0) by the hexokinase-based method and glucose test strip reading (n = 85). Campione richiesto: urina fresca raccolta al mattino, dopo aver scartata quella della notte. xref bicchiere di plastica monouso). 059/21.34.280, nelle Mattenheimer H, Adams EC. Citologia nasale. 1. S. C. Medicina di Laboratorio MODULO RACCOLTA URINE 24 ORE Revisione n.0 del 27/08/2017 PROCEDURE PER LA RACCOLTA DELLE URINE NELLE 24 ORE 1. L'esame delle urine richiesto per la certificazione di idoneità sportiva agonistica deve essere effettuato prima della data della visita (entro i 30 giorni precedenti) utilizzando l'apposito modulo fornito dalla Società Sportiva per l'accesso al laboratorio analisi e seguendo le istruzioni. The two outer solid lines represent the 95% prediction interval around the regression line. The correlation between WBC data (x) and leukocyte esterase measurements (y) is shown in Fig. /Contents 17 0 R Because microalbuminuria is defined as excretion of 30–300 mg of albumin/24 h (20–200 μg/min, or 30–300 μg/mg of creatinine) (20), the protein field result allows identification of microalbuminuria cases in contrast to the classic reading and reporting of the strips, which can detect only albumin concentrations 150–200 mg/L or higher (21)(22). Albumin in urine was measured immunonephelometrically on 220 randomly selected samples with use of commercially available Behring antibodies on a Behring Nephelometer II analyzer (Dade Behring) standardized against the widely accepted WHO/College of American Pathologists Certified Reference Material 470. Langlois M, Delanghe J, Steyaert S, Everaert K, De Buyzere M. Automated flow cytometry compared with an automated dipstick reader for urinalysis. Particles that cannot be classified in one of the former categories are counted as “other cells”. It has been extensively evaluated for urinalysis (9)(10)(11)(15) as well as for cerebrospinal fluid analysis (16). ATTENZIONE: Con questo servizio si accede direttamente alla sala prelievi (per prelievo sangue o tamponi + eventuale campione biologico).Per la consegna di solo materiale biologico (urine, feci, liquido seminale, etc..) è necessario utilizzare il servizio "SALTA LA … Urine puternic acide (pH < 4,5) se produc in procesele maligne (are loc distrugerea crescuta de proteine), febra, diaree abundenta, acidoza diabetica sau metabolica. The turnaround time was short enough (2–4 h) not to affect the readings: time intervals can influence results of automated test strip analysis, especially leukocyte and erythrocyte ratings (19). Kouri T, Kähkönen U, Malminiemi K, Vuento R, Rowan M. Evaluation of Sysmex UF-100 urine flow cytometer vs chamber counting of supravitally stained specimens and conventional bacterial cultures. • Apporre il timbro del C. Prelievi presso cui è stato accettato il campione. It must be noted, however, that the sensitivity (86%) may be inadequate for patients with known diabetes in whom physicians do not want to miss microalbuminuria in their annual testing. The two outer solid lines represent the 95% prediction interval around the regression line. Urine per esami di citologia urinaria raccolte Castelfranco Emilia. We also wanted to explore the possibilities of combining the two novel methods, particularly in the analysis of erythrocytes, leukocytes, and glucose, which are of major clinical importance. Agreement was fair between the flow cytometric RBC data (x) and the URISYS 2400 hemoglobin reflectance measurements (y) for counts above the upper reference limit (25 × 106 RBC/L on UF-100). Gambke B, Kouri T, Kutter D, Nagel D, Schmid R, Scholer A. Miditron® multicenter evaluation. The role of automated urine particle flow cytometry in clinical practice. Glucose was measured by a hexokinase method standardized against Standard Reference Material 917a and Standard Reference Material 965 (n = 85), and total protein by a pyrogallol red method (17) (n = 129) with Standard Reference Material 917a as a standard and commercially available reagents for both measurement procedures (Roche) on a Modular P system (Roche). Prendere nota dell'ora (ad esempio ore 7). Istruzioni per la raccolta delle urine per l'esame delle 24 ore. TAMPONI URETRALI. La documentazione di riferimento per l‟esecuzione dell‟esame urine dovrebbe comprendere una accurata descrizione della fase preanalitica, analitica e post-analitica. Methods: We compared URISYS 2400 (Roche) quantitative reflectance data with data from the UF-100 (Sysmex) and biochemical data for 436 nonpathologic and pathologic urine samples. Because microalbuminuria is characterized by large intraindividual variability, an ordinal-scale answer might be misleading. 0000007901 00000 n Esame delle urine: si cambia Published in: La Rivista Italiana della Medicina di Laboratorio, July 2017 DOI: 10.1007/s13631-017-0157-7: Authors: Rudi Ravasio, Giulia Previtali, Francesca Nembrini, Maria Grazia Alessio View on publisher site Alert me about new mentions. 0000016847 00000 n The ratio of hemoglobin reflectance to RBC count was constant when osmolality was >190 mosmol/L. As shown by our multiple regression model and dilution experiments, the hemoglobin field reflectance test is not influenced by dilution effects. Recently, the URISYS 2400 automated urine test strip analyzer (Roche Diagnostics) was introduced. 3. Does antihypertensive therapy protect the kidney in essential hypertension?. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. When urinary WBC counts were higher than the upper reference limit (25 × 106 cells/L), the following regression equation was obtained: y (%) = 83.7 −15.4 logx (106 cells/L); Spearman r = −0.688; P <0.001. The protein test (n = 220) detected 86% (95% confidence interval, 78–92%) of samples with <30 mg/L albumin with a specificity of 84% (95% confidence interval, 76–91%). It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide, This PDF is available to Subscribers Only. 0000016709 00000 n The dashed lines represent the lower limit of detection (− − −) with the 2 SD limits (- - - -). Delanghe JR, Kouri TT, Huber AR, Hannemann-Pohl K, Guder WG, Lun A, et al. 0000016774 00000 n Evaluation of the Sysmex UF-100 automated urinalysis analyzer. Additional dilution experiments were carried out. Title: Microsoft Word - Note informative Sito CdC Rizzola.doc Author: pichetti Created Date: 3/18/2020 4:11:07 PM As is the case for WBCs, the lower limit of detection for RBCs is acceptable (8 × 106/L). • Applicare a lato un’etichetta con il codice a barre. Citologia per espettorato. Joris Penders, Tom Fiers, Joris R Delanghe, Quantitative Evaluation of Urinalysis Test Strips, Clinical Chemistry, Volume 48, Issue 12, 1 December 2002, Pages 2236–2241, https://doi.org/10.1093/clinchem/48.12.2236. The principle is based on argon laser flow cytometry. Provetta a fondo conico, trasparente, fornita dal Laboratorio/Centro Prelievi o acquistata in Farmacia. Ce putem intelege din rezultatul acestei analize medicale: Nu este nici o problema grava acolo. %PDF-1.4 Various low- and high-molecular mass inhibitors have been found in urine (28)(29). 0000017284 00000 n Controlla la creatinina e Ph. Fair agreement was obtained between UF-100 and test strip reflectance data for erythrocytes and hemoglobin (r = −0.680) and leukocytes and leukocyte esterase (r = −0.688). Barrare la sede nella quale si effettua la visita Via Alessandrini, 6 Consegnare il presente modulo e la provetta con le urine (almeno 4 giorni lavorativi prima della visita) presso uno dei seguenti Centri Prelievo nei giorni e negli orari indicati. <> No effect of conductivity on the leukocyte esterase field was noted. La provetta contenente le prime urine della mattina deve essere portata e consegnata dall'utente o da suo? BREATH TEST. Higher correlations were observed for biochemical and test strip data comparing protein and albumin (r = −0.825) and glucose data (r = −0.851). Leukocyte esterase reflectance (y; %) = 83.7 − 15.4 log(WBC count; 106 cells/L); Spearman r = −0.688; P <0.001. Viberti GC, Jarrett RJ, Wiseman MJ. Orsonneau JL, Douet P, Massoubre C, Lustenberger P, Bernard S. An improved pyrogallol red-molybdate method for determining total urinary protein. In this study, we compared the URISYS 2400 automated strip reader with the Sysmex UF-100 flow cytometer to evaluate the possible value of quantitative test strip data in a urinary expert system. Kutter D. The urine test strip of the future. <]/Prev 39925>> The dashed lines represent the lower limit of detection (− − −) with the 2 SD limits (- - - -). Sacks DB, Bruns DE, Goldstein DE, Maclaren NK, McDonald JM, Parrott M. Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus. Hemoglobin reflectance (y; %) = 78.2 − 19.4 log(RBC count; 106 cells/L); (Spearman r = −0.680; P <0.001. Visualizza la pagina con l'elenco dei punti prelievo aggiornata. Ben-Ezra J, Bork L, McPherson RA. Modulo di prenotazione. The reflectance value, expressed as a percentage within a range from 100% (white) to 0% (black), is therefore inversely related to the concentration of the analyte in the sample. Screening school children for albuminuria, proteinuria and occult blood with dipsticks. Mendeley readers. Istruzioni per esecuzione esame urine per idoneità sportiva agonistica Compilare il modulo sotto allegato necessario per l'accettazione del campione di urina . Because RBCs tend to lyse in urine, we investigated the linearity of the hemoglobin test strip pad. Test strip analysis plays an important role in urinalysis as such, and the value of test strip urinalysis as a screening method has been thoroughly demonstrated (1). <> Peele J, Gadsden R, Crews R. Semi-automated vs. visual reading of urinalysis dipsticks. The higher the analyte, the higher the color intensity and, thus, the lower the reflectance. Viene fornito il contenitore chiuso (capacità 3 litri) ed una provetta sottovuoto tappo bianco. Tutela della salute nelle attività sportive, tel. Of all the diagnostically relevant urinary markers, only albumin and transferrin accept protons well, so the potential error of missing Bence Jones proteinuria remains when test strip screening strategies are used in proteinuria (21), although many cases presenting with Bence Jones or tubular proteinuria show microalbuminuria (23). The two outer solid lines represent the 95% prediction interval around the regression line. Data are expressed in an ordinal scale (as “normal”, “negative”, “positive”, or as nominal concentrations) on the reports, but (quantitative) reflectance data can be downloaded to floppy disks. (Insieme con il test viene fornito il contenitore urina da 90ml e un modulo in triplice copia).Questo Test Antidroga ha un sistema di stampa del risultato tramite il suo Strumento con software, oppure il risultato del test può essere anche scansionato o fotocopiato. 0000017620 00000 n Pugia MJ, Lott JA, Kajima J, Saambe T, Sasaki M, Kuromoto K, et al. We investigated the possible use of these meters to complement flow cytometry of urine and compared reflectance readings with quantitative determinations of urinary glucose and microalbumin. endobj We also found good agreement between the hexokinase-based glucose method (x) and glucose test strip reflectance reading (y): y (%) = 53.9 − 19.3 logx (mmol/L); Spearman r = −0.851; P <0.001. Because of the procedure for applying the urine to the test strips on the URISYS 2400, improper dipping is no longer a problem, nor is confusion about sample identification or urine contamination caused by dipping the strip in the tube, which potentially leads to interferences with chromatographic methods (31). Scaderi anormale. 0000000015 00000 n x��]��q�\t�I�W�(v�q.�'{G��|�#�*�|I��V*%�4��HJ�$W����0 z�=�K�,�'K%���h�t����~�dT2�q��nrƪ�����?>�N�dn�~�����9Nn�n���31�u����� s0��f�j�}��0o��ٹ�vV:L�n�nw�~t9G/�}�-��?���zk��v����e���;�9����~�Q�x���K�#�q�xf�����˃&��x�h]�w��vJ�>Z�ڇ�B~Wٺ��7����fZ�]��U�2�� ��n�n� urine delle successive 24 ore, comprese quelle delle ore 7.00 del mattino successivo; durante tutto il periodo di raccolta mantenere il contenitore chiuso in luogo fresco e su superficie sicura, rigorosamente al buio e avvolto in carta stagnola, lontano dalla portata Ottomano C. Tecnologia ed automazione dell’ esame urine quantitativo. Diagnostic accuracy was assessed by ROC analysis using commercially available statistical software (MedCalc®). The UF-100 flow cytometer automatically determines the conductivity, so we performed multiple regression analysis on the study population, calculated in a regression model the hemoglobin reflectance vs RBCs and conductivity, and found that the t-value (as expected) for RBCs was −8.442 (P <0.0001). The reproducibility of (semi)automated readings is at least as good as visual readings (2), but most authors find the analytical, clinical, and labor cost-saving advantages of (semi)automated vs visual reading to be obvious (3). European urinalysis guidelines. SPERMIOGRAMMA. The correlation coefficient for the glucose signal with the routine hexokinase-based method was −0.851. Similarly, protein reflectance data and total protein measurements correlated well (Spearman r = −0.921; P <0.001), which is expected as long as the major protein is albumin. Valori scazute ale pH-ului urinar (deci urine acide) se intalnesc in: Reumatismul poliarticular cronic; (11) reported disagreement in erythrocyte counts between the UF-100 and the hemoglobin test strip reaction in 6.5% of cases. The strips include reagent pads for ordinal scale reporting of relative density, pH, leukocyte esterase, nitrite, protein, glucose, ketones, urobilinogen, bilirubin, and hemoglobin/myoglobin. Because microalbuminuria is generally regarded as an excellent marker for assessing early renal damage in common conditions such as diabetes and hypertension (4)(5)(6)(7) and as an early predictor of preeclampsia during pregnancy (8), the availability of highly sensitive test strip readers opens interesting perspectives for assessing this phenomenon. Modificato il 18 aprile 2017 . Specific gravity (refractometry based) and clarity are measured in a flow cell, and color is rated with a specific algorithm against the blank pad on the test strip. The automation of sediment analysis using a new urine flow cytometer (UF-100). 0000016642 00000 n The reproducibility of (semi)automated readings is at least as good as visual readings (2), but most authors find the analytical, clinical, and labor cost-saving advantages of (semi)automated vs visual reading to be obvious (3). 0000001055 00000 n The dashed lines represent the lower limit of detection (− − −) with the 2 SD limits (- - - -). 1. 0000007964 00000 n Mattenheimer H, Adams EC. In our study correlating test strip data with urinary flow results, r was −0.69 between the flow cytometric WBC count and the leukocyte esterase reaction, although the presence of esterase inhibitors in urine and severe proteinuria might negatively affect test results for leukocyte esterase (1)(27). 17 0 obj Ultimo aggiornamento: 19 Luglio 2019 Condividi. 0000017492 00000 n As is the case for test strips in general, the method is based on the so-called “indicator error” principle, in which proton exchange between the indicator on the strip and the proteins in the solution produces a color change from yellow to green-blue. Liquido seminale per citologia. 0000000752 00000 n MODULO DI RICHIESTA ESAMI CITOLOGIA ED ISTOLOGIA via Ettore Majorana 10 - 25020 Flero (BS) - T. 030.6950238 - F. 030.6950239 - P.IVA 03005430982 CITOLOGIA [ ] Esame Citologico Singola Lesione 3034 [ ] Esame Citologico Midollo + Emocromo 3035 [ ] Esame Citologico LCR + Chimico Fisico 3036 [ ] Esame Citologico Liquido Da Versamento 3037 14 0 obj Moreover, a 16% false-positive rate may be unacceptable for screening. TEST DI MEARES. Department of Clinical Chemistry University Hospital Ghent. Access to the instrument’s raw data theoretically allows a higher analytical sensitivity for several analytes. 0000016556 00000 n Two discrepancies (0.9%) were found in which the test strips on both automated strip readers overestimated the urinary albumin concentration by >10-fold. The intensity of the reaction color of the test pad is detected by measuring the percentage of light reflected from the surface of the test pad. NOTA: La prenotazione deve essere richiesta almeno due giorni prima del giorno prescelto per l’esame. Modulo di richiesta: deve riportare Nome, Cognome, data di nascita e codice fiscale, struttura e/o Medico richiedente nonché i dati clinici essenziali (ematuria, litiasi, precedenti neoplasie vescicali, urine da catetere, da ureterostomia, da vescica ileale).. Modulo Esame Citologico. L’analisi del sedimento urinario valuta inoltre la presenza di cellule, cristalli e cilindri. Colombo JP eds. Glucose reflectance (y; %) = 53.9 − 19.3 log(glucose; mmol/L); Spearman r = −0.851; P <0.001). This leads to improved elimination of occasional errors in the WBC and RBC counting channels of the flow cytometer (11). The peroxidase-like activity of the hemoglobin-haptoglobin complex. Westgard JO. 0000029025 00000 n Deferrari G, Repetto M, Calvi C, Ciabattoni M, Rossi C, Robaudo C. Diabetic nephropathy: from micro- to macroalbuminuria. 0 0000017717 00000 n 1. Secrezioni mammarie. Modde H, Rodriguez S. Urin Mikrobiologie. Results: Reproducibility of the reflectance signal was good for high- and low-concentration urine pools for protein (0.8% and 0.9% and 1.5% and 2.2% within and between runs, respectively), leukocyte esterase (1.1% and 1.0%; 5.1% and 1.2%), hemoglobin (1.7% and 1.1%; 8.9% and 1.1%) and glucose (2.1% and 0.5%; 6.5% and 2.3%). Alkalinization of urine gives rise to erroneous results in particle counting. Regression equation for urinary WBC counts above the upper reference limit (25 × 106 cells/L; n = 132). When monitoring our samples, we found a median pH of 6.5 (95% interval, 5–8). Conclusions: In urine test strip analysis, quantitative hemoglobin and leukocyte esterase reflectance data are complementary with flow cytometric results and glucose and albumin results. Urine per esami di citologia urinaria Vignola. Screening and management of microalbuminuria in patients with diabetes mellitus: recommendations to the Scientific Advisory Board of the National Kidney Foundation from an ad hoc committee of the Council on Diabetes Mellitus of the National Kidney Foundation. Examenul de urina este un test de diagnostic si ajuta la identificarea substantelor si a materialului celular din urina, asociate cu diferite tulburari metabolice si renale. stream Van Acker J, Delanghe J, Langlois M, Taes Y, De Buyzere M, Verstraete G. Automated flow cytometric analysis of cerebrospinal fluid. Winkens RAG, Leffers P, Degenaar CP, Houben AW. 0000028490 00000 n ECLM. The detection limit of the URISYS 2400 protein assay was 25 mg/L if restricted to albumin alone and total protein was not considered. Bennett PH, Haffner S, Kasiske BL, Keane WF, Mogensen CE, Parving HH, et al. The UF-100 automatically detects and counts red blood cells (RBCs), white blood cells (WBCs), bacteria, yeast cells, crystals, epithelial cells, small round cells, sperm cells, and casts. Per la raccolta delle urine delle 24 ore è necessario seguire scrupolosamente questa procedura: Il mattino del giorno d'inizio raccolta, scartare tutta l'urina della prima minzione mattutina, svuotando completamente la vescica nel WC. ESAME URINE PER IDONEITA’ AGONISTICA MEDICINA DELLO SPORT ... Consegnare il presente modulo e la provetta con le urine (almeno 4 giorni lavorativi prima della data della visita medico sportiva ) presso uno dei seguenti Centri Prelievo, nei giorni e negli orari indicati. 0000017558 00000 n For WBCs, we found a lower limit of detection of 19 × 106 cells/L (CV = 1.2%). trailer Of course, the frequency of erroneous results depends on the proportion of pathologic samples and on the preanalytical handling of samples. Urine test strip analysis was performed with the automated URISYS 2400. Per effettuare l’esame urine compilare il presente modulo in ogni sua parte IN STAMPATELLO:!"!" Ce intelegem din rezultatul examenului de urina? 0000017589 00000 n The inhibitory effect of urine on the peroxidase-like activity of hemoglobin and on horseradish peroxidase. Multiple regression analysis was used to investigate a model relating leukocyte esterase and hemoglobin field reflectance. Nulla è dovuto a pagamento dell’esame urine se l’interessato è minorenne o disabile : se maggiorenne, il pagamento di tale esame è compreso nell’importo dovuto per la visita. The hemoglobin measurement is based on the peroxidase principle. The change from urine microscopy to urinary flow cytometry has been accompanied by a significant decrease in imprecision (15). This offers several possibilities: (a) The sensitivity for albumin may allow affordable screening for microalbuminuria, particularly in patients with undiagnosed renal damage. In conclusion, quantitative urine test strip analysis provides reliable data on WBCs, RBCs, glucose, and albumin. Od krvi se razlikuje po tem, da ne vsebuje krvnih celic (eritrocitov, levkocitov, trombocitov) in makromolekularnih snovi – beljakovin. 0000029149 00000 n >> Whelan PV, McGill C, Ng K. Evaluation of the BMC Chemstrip urine analyzer and Chemstrip 10 UA test strip with emphasis on comparison with analytical, chemical, physical, and microscopic methods [Abstract]. In this study, we wanted to investigate the performance of quantitative urinary test strip analysis.

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